Affinity Chromatography as a Key Tool to Purify Protein Protease Inhibitors from Plants
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Assessment and partial purification of serine protease inhibitors from Rhipicephalus (Boophilus) annulatus larvae Avaliação e purificação parcial dos inibidores da serina protease de larvas do Rhipicephalus (Boophilus) annulatus
Ticks are rich sources of serine protease inhibitors, particularly those that prevent blood clotting and inflammatory responses during blood feeding. The tick Rhipicephalus (Boophlus) annulatus is an important ectoparasite of cattle. The aims of this study were to characterize and purify the serine protease inhibitors present in R. (B.) annulatus larval extract. The inhibitors were characterize...
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Activation-Induced Deaminase (AID) protein functions in class switch recombination and somatic hypermutation in mammalian cells. The AID protein deaminates cytidine sites in single stranded DNA. We designed and constructed an expression plasmid that encodes AID as a recombinant fusion protein. Our construct includes an N-terminal maltose binding protein as a solubility and expression tag follow...
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تاریخ انتشار 2017